http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112695047-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6427 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8243 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8247 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1229 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-04027 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-89 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-13 |
filingDate | 2021-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112695047-B |
titleOfInvention | Method for producing fucoxanthin by using mutant strain expressing exogenous pyruvate phosphate dikinase in phaeodactylum tricornutum |
abstract | The invention provides a method for producing fucoxanthin by a mutant strain expressing exogenous pyruvate phosphate dikinase in phaeodactylum tricornutum, in particular to a method for optimizing algae preference codons of an original CyPPDK gene to obtain a new CyPPDK gene, wherein the nucleotide sequence is shown as SEQ ID No. 7; the constructed shuttle expression vector is named pPha-fcp-PPDK; introducing the cells into a host algae cell Phaeodactylum tricornutum by an electrotransformation mode, and screening a transformant to obtain a transgenic phaeodactylum tricornutum strain over-expressing pyruvate phosphate dikinase; compared with the non-optimized transformant, the optimized transformant carrying out phosphorylation sites on CyPPDK gene has the advantages that the biomass accumulation is improved by 33.3 percent, the content of EPA in oil is improved by 11.5 percent, and the yield of fucoxanthin is improved by 21.2 percent. |
priorityDate | 2021-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 447.