| abstract |
The invention relates to a genetically engineered bacterium with high yield of cypressotrienol, a construction method and application thereof, and belongs to the field of bioengineering. In order to achieve high-yield, high-efficiency, and low-cost synthesis of femmetrienol, the present invention connects the tobacco femmetrienol synthase gene CBTS2a, whose nucleotide sequence is shown in SEQ ID NO. 1, to an expression vector, and then transforms it into From the engineering bacteria using the MVA pathway and the GGPP high-energy synthesis pathway, a genetically engineered bacteria for producing cerebrotrienol is obtained. The genetically engineered bacteria of the present invention can be fermented and cultured for 46 hours, and the yield of cepatrienol can be as high as 500 mg/L. The genetically engineered bacteria of the present invention can directly obtain high-yield and high-purity cerebrotrienol from the bacteria body, and has a good industrial application prospect. |