http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112680431-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
filingDate | 2021-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112680431-B |
titleOfInvention | DNA library preparation method based on fragmenting enzyme |
abstract | The invention provides a novel fragmenting enzyme which can randomly break a genome into fragments of about 50-150bp at high efficiency. The invention also provides a one-step method for quickly constructing the DNA library. The database building method comprises the following steps: the steps of DNA (genome or other large-fragment dsDNA sequences) fragmentation, end-to-end A addition and linker ligation are integrated into one step, a one-tube reaction is carried out, and a DNA library is obtained through purification and enrichment. The one-step library construction method can efficiently and quickly obtain a high-quality DNA sequencing library. |
priorityDate | 2021-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 551.