http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112662679-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-74 |
filingDate | 2020-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112662679-B |
titleOfInvention | A kind of peach fruit polygalacturonase inhibitory protein PpPGIP1 gene and its cloning method and application |
abstract | The invention discloses a peach fruit polygalacturonase inhibitory protein PpPGIP1 gene, a cloning method and application thereof, and is characterized by the peach fruit polygalacturonase inhibitory protein PpPGIP1, the nucleotide sequence of which is as shown in SEQ ID NO: 1 The amino acid sequence of the protein encoded by the peach fruit polygalacturonase inhibitory protein PpPGIP1 is shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) Extracting the total RNA of peach fruit and reverse transcribing it into cDNA as Template; (2) Design primers according to the gene sequence of PpPGIP1; (3) PCR amplification: The amplification product of PpPGIP1 gene is obtained by PCR amplification. The advantage is that there is a protein interaction between PpPGIP1 and PpVIN2, which can effectively inhibit the expression of PpPGIP1 in peach It can significantly reduce the activity of acid invertase PpVIN2 and reduce the decomposition of sucrose. |
priorityDate | 2020-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.