abstract |
The present invention uses xylanase GH11-xylanase as a template, adopts molecular biological technology to carry out amino acid mutation, and obtains a class of mutants with high catalytic efficiency, including one or more site mutations in W34F and Q35H, and may further have N36Y mutation. The mutant of the invention has the excellent property of high catalytic efficiency, is beneficial to reduce the use cost of xylanase, improves the conversion efficiency of producing xylo-oligosaccharide by using the enzyme, and has broad industrial application prospects. |