| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_311159e9645e7b312c4272609ac1e08d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-14043
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-552
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2469-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-183
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24352
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-105
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24334
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24322 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 |
| filingDate |
2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87c701658e4a47c5a314a0bb445636fc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17d32bf8e6fe9f6320665ffebfc1a5bc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52b98e3d5f9b25b489217f18c90cbbee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cca83442d47dcd47d9ffb47b4fceaee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2050d800952debf7dfba27eba204e43c |
| publicationDate |
2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112646840-A |
| titleOfInvention |
Method and application of a baculovirus expression system for preparing bovine viral diarrhea virus E2 protein |
| abstract |
The invention belongs to the technical field of veterinary biological products, in particular to a method and application for preparing bovine viral diarrhea virus E2 protein by a baculovirus expression system. The E2 gene of the BVDV‑LC strain was connected to the pFastBac1 recombinant baculovirus transfer vector, and the recombinant baculovirus vector rBacmid‑E2 was obtained by homologous recombination in E. coli, and the first-generation recombinant baculovirus P1 was obtained after transfection into Sf9 insect cells , continue to infect Sf9 insect cells with the P1 generation virus to obtain the second generation recombinant baculovirus P2 with high infectivity, and then expand the P2 generation virus and purify to obtain the E2 protein. Using the E2 protein prepared by the present invention as a diagnostic antigen and a subunit vaccine can show better advantages, and can be used as a candidate antigen for the development of vaccines and ELISA kits. |
| priorityDate |
2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |