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filingDate 2021-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112630429-B
titleOfInvention Urine helicobacter pylori antibody detection kit and preparation method thereof
abstract The invention discloses a reagent kit for detecting a helicobacter pylori antibody in urine and a preparation method thereof. The kit comprises a reagent strip positioned on a bottom plate, wherein the reagent strip comprises a sample pad, a fluorescent microsphere combination pad, a nitrocellulose membrane and a water absorption pad in sequence from a sample adding end; the sample pad is coated with a biotinylated helicobacter pylori antigen B; the fluorescent microsphere bonding pad is coated with anti-human IgG labeled by the time-resolved fluorescent microsphere and helicobacter pylori antigen A labeled by the time-resolved fluorescent microsphere; one end of the nitrocellulose membrane, which is close to the fluorescent microsphere binding pad, is provided with a detection line coated with avidin; and one end of the nitrocellulose membrane close to the water absorption pad is provided with a quality control line coated with goat anti-mouse IgG. The invention completes the triple amplification of detection signals and improves the sensitivity of the reagent. The invention realizes the detection of the helicobacter pylori antibody from urine, and has the advantages of convenient sampling, complete non-wound and high detection sensitivity.
priorityDate 2021-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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