http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112630422-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c0582e46196a056fd2f7337b6bf049a |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70514 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate | 2020-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b0cbf2e46005f943da3802f5179d2fd |
publicationDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112630422-A |
titleOfInvention | Method for improving signal-to-noise ratio of antibody and fluorescent protein directional coupling label |
abstract | The invention relates to a method for improving the signal-to-noise ratio of an antibody and a fluorescent protein directional coupling label, which comprises the following steps: step 1) reacting PE with a first crosslinking agent; step 2) reacting the antibody with a second cross-linking agent; step 3) carrying out cross-linking reaction on the PE cross-linked in the step 1) and the antibody cross-linked in the step 2); wherein the first cross-linking agent is Sulfo-S-HyNic or S-HyNic, and the second cross-linking agent is Sulfo-S-4FB or S-4 FB; or the first cross-linking agent is Sulfo-S-4FB or S-4FB, and the second cross-linking agent is Sulfo-S-HyNic or S-HyNic; when the method is used for immunodetection, the positive signal is strong, and the signal-to-noise ratio is high. |
priorityDate | 2020-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.