| abstract |
The invention discloses a primer pair, a kit and a detection method for detecting riemerella anatipestifer. The sequences of the primer pair are shown as SEQ ID NO.1 and 2; can amplify a target band with the size of about 330 bp; the detection method comprises the following steps: (1) extracting sample DNA for later use; (2) the primer pair of claim 1 is used for PCR amplification, and the amplification result is detected, if a specific band with the size of about 330bp can be obtained by amplification, the sample contains the riemerella anatipestifer. The invention establishes a specific PCR detection method of riemerella anatipestifer, so thatThe method is used for PCR amplification, target fragments with the size of 330bp can be amplified from the DNA of different RA strains, the minimum detected amount is 47pg, and the minimum bacteria liquid concentration capable of being detected is 5.7 multiplied by 10 4 CFU/mL, good specificity, high sensitivity and good repeatability. |