| abstract |
The invention belongs to the field of biotechnology, and particularly relates to a primer composition for microsatellite instability detection, a kit and application. The present application provides a primer composition for microsatellite instability detection comprising: a first primer combination, a second primer combination, a third primer combination, a fourth primer combination, a fifth primer combination, and a sixth primer combination; the primer combination is SEQ ID NO. 1-SEQ ID NO.12 in sequence. Compared with an immunohistochemistry method and an HRM method, the primer composition, the kit and the application for microsatellite instability detection disclosed by the application have the advantages of accurate and reliable result, high sensitivity and high specificity. |