http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112626152-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78160fe5924d399deb065f8cbc5ad347 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8838c0a3d73dc4722a798b1911e7e15e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2020-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3bbd96dd2c7d294e3269c7e9e3ee391 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_210b842d65f45b0f246cba02143fe9a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a511cdc89d6ffd46e5019441124b60ab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d39c976ffa8323ba3a21b59aa9906ca |
| publicationDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112626152-A |
| titleOfInvention | Preparation method of siRNA |
| abstract | The present application relates to the technical field of nucleic acid extraction, in particular to a method for preparing siRNA, the preparation method comprising the steps of: in vitro transcription to obtain dsRNA of a target gene; digesting the dsRNA of the target gene with RNase III to obtain a digested product; After gel electrophoresis of the digested product, a gel block with a nucleic acid fragment of less than 30 bp was cut; after the gel block was ground, frozen and thawed, siRNA was obtained. The preparation method has the characteristics of accuracy, effectiveness, simple operation and low cost, brings great convenience to the purification of siRNA and the research of RNAi, and has a good application prospect. |
| priorityDate | 2020-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 614.