| abstract |
The application discloses a hawthorn standard reference substance and its preparation method, detection method and application. The preparation method of the hawthorn standard reference substance includes the following steps: step 1: extracting decoction pieces of hawthorn with water, drying the water extract to obtain a dried product; step 2: extracting the dried product with methanol, and evaporating the methanol extract to dryness to obtain Evaporation to dryness; Step 3: Dissolving the evaporated dry matter in a mixture of water and an organic solvent to obtain a mixture, injecting the mixture into a high-speed inversion chromatograph, collecting the effluent for a specific period of time under specific conditions, and using The effluent is dried to prepare the hawthorn standard reference substance. The preparation method of the hawthorn standard reference substance is simple, the extracted hawthorn standard reference substance has high purity, and the hawthorn standard reference substance contains a variety of components, which can improve the quality standard of hawthorn and related medicines or preparations using hawthorn as a raw material, and improve the quality of the hawthorn standard reference substance. Product quality controllability. |