http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112608953-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-146 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N43-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D207-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-34 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-465 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D207-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01P3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N43-36 |
filingDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112608953-B |
titleOfInvention | Preparation method of anisomycin, detection method of anisomycin and antibacterial application |
abstract | The invention discloses a preparation method of anisomycin, a detection method of anisomycin and antibacterial application, wherein the preparation method of anisomycin comprises the following steps: inoculating a strain seed liquid of the streptomyces albidoflavus CK-15 strain to a seed culture medium, culturing at 28 ℃, 220rpm for 24 hours, transferring the strain seed liquid to 100ml of fermentation culture medium according to the inoculation amount of 5%, fermenting at 30 ℃ for 5 days, centrifuging the obtained fermentation liquid, and collecting a first supernatant; adjusting the pH of the first supernatant to 9.0, and extracting the first organic phase with an equal volume of ethyl acetate to the supernatant; counter-extracting the first organic phase with a volume of acid water equal to the first organic phase at a pH of 2.0, collecting the aqueous phase and adjusting the pH of the aqueous phase to 9.0; extracted with an equal volume of ethyl acetate to the aqueous phase, the second organic phase was collected and spin dried. Compared with the preparation method of the anisomycin by adopting a chemical synthesis method, the preparation method of the anisomycin has the advantages of simplicity, convenient operation and lower cost. |
priorityDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.