| abstract |
A combined saccharide feed medium for CHO cell culture and a culture medium adding method of CHO cells in view of temperature change are provided, wherein the feed medium is added at different culture temperature nodes in the culture process, the temperature nodes are 30-34-37-34-32 ℃ in sequence, and saccharide concentration and combination of feed medium 1-5 are optimized and selected, so that the method can effectively improve the improvement of foreign protein. |