http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112592958-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-904 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-99002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 |
| filingDate | 2020-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112592958-B |
| titleOfInvention | A method for the detection of D-2-hydroxyglutarate using FAD-dependent D-2-hydroxyglutarate dehydrogenase and resazurin |
| abstract | The invention discloses a method for detecting D-2-hydroxyglutarate by using FAD-dependent D-2-hydroxyglutarate dehydrogenase and resazurin, and relates to the technical field of enzymatic detection. The method utilizes FAD-dependent D-hydroxyglutarate 2-Hydroxyglutarate dehydrogenase, resazurin and PBS buffer to realize the detection of D-2-hydroxyglutarate; the FAD-dependent D-2-hydroxyglutarate dehydrogenase is AX-F ‑D2HGDH protein or P‑D2HGDH protein; the detection method of the present invention has simpler components, lower cost, and easier operation; since there is no need to add cofactors, coenzymes or diaphorase, the introduced interference is less and the sensitivity is higher, In addition, only one enzyme needs to be prepared, and there is no need to add exogenous cofactors such as NAD. At the same time, the buffer can be a lower-cost and easy-to-obtain PBS buffer, so the cost is lower. |
| priorityDate | 2020-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 569.