| abstract |
The present invention provides a hybridoma cell strain and its application. The hybridoma cell strain can secrete a monoclonal antibody of tomato spotted wilt virus, and the monoclonal antibody is used to prepare an immunogold standard quick test card. The method is as follows, step 1 , Purify tomato spotted wilt virus recombinant protein, step 2, immunize rabbits with the purified tomato spotted wilt virus recombinant protein to obtain polyclonal antibodies, step 3, immunize mice with the purified tomato spotted wilt virus recombinant protein, and then pass the cell The monoclonal antibody is prepared by fusion, cloning screening, and finally mouse ascites is prepared. After purification, the mouse anti-tomato spotted wilt virus monoclonal antibody is obtained. According to the immunogold standard quick test card, the present invention has the advantage that the immunogold standard quick test card can be applied to tomato spotted wilt virus for rapid, on-site and sensitive detection. |