http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112592881-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y206-01009 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 |
filingDate | 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112592881-B |
titleOfInvention | Engineered Bacillus subtilis for high-efficiency exogenous protein expression and high-density culture |
abstract | The invention belongs to the field of bioengineering, in particular to a genetically engineered Bacillus subtilis used for high-efficiency exogenous protein expression and high-density culture. The function of hisC gene was restored by genetic modification of histidine-deficient Bacillus subtilis to obtain a non-auxotrophic strain. Further, by knocking out the genes spoIIAC and srfAC , the obtained strains are more suitable for expressing various heterologous proteins. The non-auxotrophic Bacillus subtilis for expressing heterologous protein obtained by the present invention can provide a low-cost high-density fermentation technology, which can greatly reduce the fermentation cost and express heterologous protein at a high level. |
priorityDate | 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1083.