http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112577803-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B2503-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B2503-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-305 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B5-153 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B5-15003 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61B5-153 |
filingDate | 2020-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112577803-B |
titleOfInvention | Method for preparing turtle chromosome specimen by using peripheral blood cells |
abstract | The invention discloses a method for preparing a turtle chromosome specimen by using peripheral blood cells, belonging to the field of cytogenetics. According to the invention, the metaphase specimen of the soft-shelled turtle chromosome, which is convenient to observe and clear in image, is prepared by collecting peripheral blood of the soft-shelled turtle, and performing the steps of blood cell culture, colchicine treatment, dyeing and flaking and the like. The invention overcomes the problem of great damage to animals in the existing method for preparing chromosome specimens of aquatic animals, realizes non-destructive sampling, only needs to collect a small amount of peripheral blood of the samples, and has little influence on the survival state of the animals. The method for preparing the soft-shelled turtle chromosome is simple and rapid, the metaphase mitotic phase of the soft-shelled turtle chromosome can be obtained 72 hours after blood collection, observation is facilitated, and a favorable tool is provided for further developing relevant researches on the genome and evolution of the soft-shelled turtle and realizing more sufficient protection of the soft-shelled turtle. |
priorityDate | 2020-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.