| abstract |
A method for rapidly positioning industrial hemp character related genes belongs to the technical field of industrial hemp research. The method comprises the following steps: according to the target characters, variety selection, extreme group construction, specific site amplification fragment library construction and high-throughput sequencing, marker development, association analysis, gene annotation and real-time fluorescence quantitative PCR are carried out. According to the invention, a high-low mixed pool is constructed for F1 generation groups obtained by hybridizing parents with larger target character differences, the parents are subjected to resequencing, the high-low mixed pool is subjected to sequencing by adopting a simplified genome deep sequencing technology, a SLAF label is developed and single nucleotide polymorphism detection is carried out, marker association analysis is carried out for genotype frequency difference between mixed pools, a target character related candidate area is obtained, most plant groups can be subjected to gene localization only when the propagation is carried out to F2 generation at least, and due to the high heterozygosity characteristic of industrial hemp, the gene localization is realized through F1 generation, so that the rapid localization of industrial hemp genes can be realized in a high-efficiency and low-cost manner. |