| abstract |
The invention discloses a method for preparing a grass carp reovirus oral subunit vaccine by using a duckweed expression system. The invention can simply and efficiently express the grass carp reovirus oral subunit vaccine by utilizing a duckweed expression system, and the method comprises the steps of amplification of exogenous genes, construction of an agrobacterium plant binary expression vector, establishment of a duckweed callus genetic transformation system, and verification of the expression of the exogenous genes by using experimental technologies such as PCR, fluorescence microscopic observation, GUS staining and Western blotting. The duckweed-containing vaccine can be used for efficiently expressing the grass carp reovirus subunit vaccine, the prepared vaccine can be directly orally taken to immunize grass carps, economic loss caused by grass carp reovirus infection can be reduced, the operation is convenient, the cost is saved, and the popularization and practical application values are higher. |