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filingDate 2020-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112575013-B
titleOfInvention Application of gene GNATB as screening marker gene in resistance screening
abstract The invention discloses application of a gene GNATB as a screening marker gene in resistance screening and application thereof. The nucleotide sequence of the gene GNATB is shown in SEQ ID NO. 1. The novel screening marker gene GNATB can efficiently assist the saccharomyces cerevisiae to resist cycloheximide, thereby laying a foundation for reconstructing a biosynthetic pathway of cycloheximide and analyzing an action mechanism of the screening marker gene GNATB and cycloheximide in the later period, promoting a heterologous expression level of cycloheximide and laying a molecular biological foundation for obtaining the novel cycloheximide. In addition, the acetyltransferase gene GNATB can be developed into a high-expression and high-resistance screening marker gene, is used for screening and maintaining and culturing eukaryotic cells successfully transfected with the cycloheximide resistance gene, and is applied to the fields of molecular biology, microbiology, medicine and the like.
priorityDate 2020-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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