http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112574961-A

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filingDate 2020-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e588db28c6a491f23b885a6bf94a048e
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publicationDate 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112574961-A
titleOfInvention A kind of preparation method of cell vaccine antigen
abstract The invention discloses a method for preparing a cell vaccine antigen, which includes the steps of healthy cell passage, poisoning and liquid exchange, and antigen harvesting. In the present invention, when the cells are passaged and digested, a shaker is used instead of manually shaking bottles, which can greatly reduce labor intensity; When changing the medium, connect several glass bottles with fresh maintenance solution in series, and pump the maintenance solution in the glass bottle into the cell culture bottle through the peristaltic pump, so that the process of changing the medium is semi-automatic, which can reduce the number of operators and reduce The labor intensity of the production process improves the production efficiency, and also reduces the contamination rate of semi-finished products; when the antigen is harvested, it mainly relies on the ultrasonic transformation energy method to break the cells in the cell culture flask, and use this method to scrape the seedlings, avoiding the traditional method of scraping The shortcomings of the seedlings also save the steps of cell freezing and thawing, so that the harvesting process can be completed in one step, achieving the purpose of simplicity, safety, labor saving, and shortening of antigen production time.
priorityDate 2020-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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Total number of triples: 25.