http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112574886-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-02 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-24 |
| filingDate | 2020-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112574886-B |
| titleOfInvention | Method for separating pathogenic bacteria of periodontitis |
| abstract | The invention relates to the technical field of microbial culture, and discloses a method for separating pathogenic bacteria of periodontitis, which comprises the following steps: sampling bacteria: sampling from the slit between the teeth of a human body by adopting an aseptic dental floss rod to prepare an original sample suspension; preparation of a separation culture medium: mixing the mixed peptone, yeast extract powder, sodium chloride, agar, glucose, sodium bicarbonate, L-cysteine salt and soluble sodium pyrophosphate according to a certain proportion, adding water to a constant volume of 1L, sterilizing and cooling, adding heme, vitamin K and sterile defibrinated sheep blood according to a certain proportion, pouring the mixture into a flat plate, and cooling to obtain a separation culture medium; and (3) culturing bacteria: the original sample suspension was removed from the anaerobic incubator and cultured in the isolation medium at 37 ℃ under anaerobic conditions to produce characteristic monoclonal antibodies. The sampling method provided by the invention is simple and rapid, does not aggravate gingival bleeding and infection risks, and the provided separation culture medium has high specificity, and shortens the culture period of primary separation of periodontitis pathogenic bacteria. |
| priorityDate | 2020-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.