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inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0aa0e47397d42953e79ddeb863d7dc1b
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publicationDate 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112557563-A
titleOfInvention Method for identifying medicinal material fructus aurantii in western and Jiangxi regions
abstract The invention discloses an identification method of medicinal material fructus aurantii in western and Jiangxi provinces, which comprises the following steps: a) preparing a reference substance solution; b) preparing a test solution; c) high performance liquid chromatography determination; d) taking 15 batches of medicinal material fructus aurantii in the way of Jiangxi to respectively prepare test solution, sequentially carrying out high performance liquid chromatography determination, recording fingerprint, introducing all the fingerprints into traditional Chinese medicine chromatography fingerprint similarity evaluation system software, selecting chromatographic peaks existing in the fingerprints of different batches of fructus aurantii as common chromatographic peaks, generating standard control fingerprints, and calculating relative retention time and peak area of each common chromatographic peak; e) identifying the common chromatographic peak by adopting a liquid chromatogram-tandem mass spectrometer; f) clustering analysis and principal component analysis are adopted. The method has simple and convenient operation, good reproducibility and stability, adopts the liquid chromatography-mass spectrometry technology, defines the compound structures of all common chromatographic peaks, and can characteristically distinguish the Jiangxi genuine property area and other production areas of fructus aurantii by combining the clustering analysis and the principal component analysis.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114441680-A
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