http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112557473-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-307 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2019-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112557473-B |
| titleOfInvention | Preparation method and application of electrochemical sensor based on zirconium metal-organic framework |
| abstract | The invention relates to a preparation method of an electrochemical sensor based on a zirconium metal-organic framework and application thereof, wherein malignant glioma is one of the most fatal tumors in brain, and the early diagnosis is still technically challenging due to the complexity of carcinogenesis reasons and the obstruction of blood brain barrier. Specific exosomes secreted by malignant glioma can pass through blood brain barrier and circulate in body fluid, and have the potential of noninvasive early diagnosis. Therefore, we propose a novel label-free electrochemical biosensor based on a zirconium metal organic framework (Zr-MOF). In the method, the designed polypeptide chain can specifically capture highly expressed human Epidermal Growth Factor Receptor (EGFR) and EGFR variant (v) III mutant strain (EGFRvIII) in malignant glioma exosome. Meanwhile, Zr-MOF (MB @ Zr-MOF) wrapped by Methylene Blue (MB) is used as a chemical selection marker, and a sensitive electrochemical signal is generated through interaction with a phosphate group outside an exosome phospholipid bilayer membrane. By monitoring electroactive molecules in the Z-MOF, the concentration of exosomes can be quantitatively detected, and the clinical diagnosis application value is wide. |
| priorityDate | 2019-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.