http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112553256-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-73 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-7155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
filingDate | 2021-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112553256-B |
titleOfInvention | IL-2 receptor compound and preparation method and application thereof |
abstract | The invention discloses an IL-2 receptor compound and a preparation method and application thereof. The IL-2 receptor complex is obtained by constructing the nucleic acid sequences of the encoding fusion protein I (SEQ ID NO:1 or 7) and the fusion protein II (SEQ ID NO:3 or 9) on the same eukaryotic expression vector or different eukaryotic expression vectors respectively, transfecting mammalian cells with the obtained recombinant expression vector, and separating and purifying the transfected mammalian cells from the cell culture supernatant. The invention utilizes the genetic engineering technology, directly obtains the IL-2 receptor alpha beta gamma heterotrimer protein through recombinant expression and one-step affinity chromatography purification, and does not need in vitro assembly; the heterotrimeric protein has high affinity activity with IL-2, and can be used for in vitro research of the interaction between IL-2 and an IL-2 receptor, preparation of antibodies by immune animals, in vitro screening of antibodies and the like. |
priorityDate | 2021-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 571.