http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112553248-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8207 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2020-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112553248-B |
| titleOfInvention | Establishment method and genetic transformation method of a kind of old mangosteen genetic transformation system |
| abstract | The invention discloses a method for establishing a genetic transformation system and a method for genetic transformation of A. chinensis, belonging to the technical field of plant bioengineering. The method for establishing the genetic transformation system of the described old mangosteen tested and counted the callus-inducing ability of five explants, and determined that the callus induced by the young panicle was the best transformation receptor; For young panicle and young panicle callus, the embryogenic callus of 25d and 35d induced by the young panicle of A. chinensis was selected as the target callus, and the target callus was pretreated by two methods before bombardment: hypertonicity treatment and filter paper drying, The subculture on the bombarded callus was recovered based on dark culture conditions. Finally, the genetic transformation method of Chuancao No. 2 old awn wheat was determined by using the 25d young panicle callus as the transformation receptor and the filter paper drying and pretreatment for 2h. The method can obtain the old gnat seedlings with the regeneration efficiency of 63% and the old awn wheat positive callus with the transformation efficiency of about 40%. |
| priorityDate | 2020-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.