| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_33a62710796e131599043055d399597f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y602-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate |
2019-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_faccd3973f7262e0578f159a385d5e4f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29de4f4b87d68f527e29eeeae24d8099
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb6563e2a8d50b4570a751fb1181118e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8689c0ef3a1de9ab03101913b052551
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_378671df9d5e80a046e8e47eecd18aa4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76e7e3c93f4f76600ac6e98bc75ba267 |
| publicationDate |
2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112553132-A |
| titleOfInvention |
Optimal fermentation method of Saccharopolyspora rubrum with sucC gene knockout |
| abstract |
The invention provides an optimized fermentation method of Saccharopolyspora rubrum with sucC gene knockout. The inventors observed that the engineered bacteria had autolysis in the later stage of the fermentation process, which affected the production of erythromycin, and then through the process improvement of adjusting pH and adding ammonium sulfate in a specific time period, the red color of sucC gene knockout was effectively reduced. The autolysis of Saccharopolyspora also greatly increases the proportion of erythromycin A in the total erythromycin product of the genetically engineered bacteria. In the case of using synthetic (type) medium, the erythromycin A of the present invention has high yield and high purity, and is suitable for industrial application. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113174418-A |
| priorityDate |
2019-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |