http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112552376-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 |
| filingDate | 2020-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112552376-B |
| titleOfInvention | A kind of method of purifying vilacapide |
| abstract | The invention discloses a purification method for solid-phase synthesis of veracatide, which belongs to the technical field of polypeptide medicine preparation. In this method, the solid-phase synthesized crude sample of veracatide is first dissolved in water; the crude peptide solution is added into an equal volume of acetonitrile solution and saturated sodium chloride solution to separate out, and the obtained solid is dissolved in water and then filtered, and a C18 reverse-phase chromatographic column is used to obtain 0.2% hydrochloric acid is phase A, acetonitrile is phase B, and a sample with a purity greater than 99.00% is obtained by gradient elution; the sample uses a C18 reverse-phase chromatographic column, with 50mmol/L ammonium chloride solution as phase A, acetonitrile as phase B, and the gradient is 5%‑5% to complete ion exchange, and then use 0.1% hydrochloric acid aqueous solution as phase A, acetonitrile as phase B, first gradient equilibrium, and then gradient elution to obtain the salted sample; the salted sample is concentrated under reduced pressure, freeze-dried to obtain vitamin Lacatide final product. The method of the invention greatly improves the processing capacity, shortens the production period, is suitable for industrialized large-scale production, improves the yield and reduces the production cost. |
| priorityDate | 2020-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.