| abstract |
The invention discloses a base editor. The base editor is a PX459 recombinant plasmid. The PX459 recombinant plasmid includes a PX459-U6-sgRNA-CBh-T2A-PURO vector backbone and rAPOBEC1 (W90Y+R126E; YE1) -SpCas9n(D10A)-UGI nucleotide sequence. The preparation method of the base editor is simple, and in the application, a single plasmid is used for cell transfection operation, which can not only improve the gene editing efficiency, but also specifically mutate cytosine C at the target site into thymine T, Moreover, it has no effect on the bases of non-target sites. The base editor has a wide range of applications, not only can be applied to gene editing of pig somatic cells, but also to gene editing of economic animal cells such as cattle and sheep. |