| abstract |
The invention discloses an L-isoleucine-producing recombinant strain based on the YH66_08550 gene and a construction method and application thereof. In the present invention, by knocking out the YH66_08550 gene, it is found that the product encoded by the gene has an impact on the L-isoleucine production capacity, and a recombinant strain is obtained by introducing a point mutation in the coding sequence, or increasing the copy number or overexpression of the gene, The point mutation is to mutate the 607th base of the YH66_08550 gene sequence from guanine (G) to adenine (A), and to change the 203rd glutamic acid of the encoded corresponding amino acid sequence to lysine. The obtained strain is favorable for the production of high concentrations of L-isoleucine compared to the unmodified strain. |