http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112522419-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2020-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112522419-B |
| titleOfInvention | Double PCR method for detection of parthenogenetic and hermaphroditic strains of Liriomyia wanshii |
| abstract | The present invention relates to a rapid PCR detection method for two strains of Liriomyza wandanii and specific COI primers used therefor. According to the specific sequences of mitochondrial DNA of two strains of L. wanyi, the method designs specific primers through specific sites, and finally establishes a pair of COI primers for the specific detection of L. wanyi. The primer pair includes primers DWAlgdCF: 5'-TTTGGTTTAAT CTCTCAC-3'; and DWlgdCR: 5'-ATGTAAAACAATATC AATTGAAGA-3'. One-step duplex PCR primers are universal primer COISF, specific primer DWAlgdCF and specific primer DWlgdCR. The successfully established double PCR system can also have a good detection effect on the DNA of eggs, larvae, pupae, female adults and male adults of the bisexual strains of Liriomyia wandanii, as well as the mixed DNA of the two strains in the same worm state. . The method can quickly and effectively identify one of the two strains under the background that it is difficult to accurately identify the two strains from the morphology. The application of the method greatly saves time and money, and can obtain reliable results. |
| priorityDate | 2020-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.