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filingDate 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f18093e1850f9e9636fcc7a021acd8a
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publicationDate 2021-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112522383-A
titleOfInvention A kind of preparation method of miRNA marker of nonalcoholic steatohepatitis
abstract A method for preparing a miRNA marker of non-alcoholic steatohepatitis, the invention relates to the technical field of non-alcoholic steatohepatitis research, and 2 qualified human NASH-related model miRNA sequencing data sets and 7 miRNA sequencing datasets of eligible mouse NASH-related models were downloaded, and then miRNA differential expression analysis was performed separately and the intersection was obtained; a methionine choline-deficient diet-induced mouse NASH model was constructed, and the weight of the mice was recorded; The total RNA of mouse liver tissue was reverse transcribed; the obtained reverse transcription product was used as a template to detect the differentially expressed miRNAs in both human and mouse NASH; the miRNA expression results were compared, and the expression trends were selected to be consistent and the expression The two miRNAs with the most obvious differences were used as markers for judging nonalcoholic steatohepatitis.
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