http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112522382-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4812ed1bb05682aba2e6bcc92e638ca1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8956f731ad0df736adcff2467d257206 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2a27bc99068fc021514f4d14086c7d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a40a11466e0300e29817afbf8a671c08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a25b9d478cb0c45a70c4947584e0617 |
| publicationDate | 2021-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112522382-A |
| titleOfInvention | Y chromosome sequencing method based on liquid phase probe capture |
| abstract | A method for sequencing Y chromosome based on liquid phase probe capture comprises the following steps: s1, extracting and fragmenting genome DNA; s2, performing end repair on the fragmented DNA; s3, adding sequencing connectors at two ends of the DNA fragment and purifying a connection product; s4, amplifying the fragment DNA connected with the joint by PCR to perform library amplification and purifying; s5, controlling the quality of the library; s6, selecting and optimizing a probe area, and designing a local intensive probe; s7, carrying out library hybridization reaction on the DNA library and the probe to capture a target DNA fragment; s8, separating the captured target DNA fragments; and S9, carrying out high-throughput sequencing on the separated target DNA fragment. The invention utilizes the liquid phase probe capture technology, selects and optimizes the probe region, has longer sequencing length of the Y chromosome, can test more mutations, and realizes the capture sequencing of the 20M region of the human Y chromosome. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114464261-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113930490-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114464261-B |
| priorityDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP22629 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID171548 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419555088 |
Total number of triples: 23.