http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112522174-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-045 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112522174-B |
| titleOfInvention | A method for knocking out negative regulatory protein genes to increase the level of acarbose fermentation |
| abstract | The invention discloses a method for knocking out a negative regulation gene to improve the fermentation level of acarbose. By adopting the method of the present invention, the negative regulatory protein genes ACPL_5445 and ACPL_3989 are respectively knocked out in the actinomycete mobilis to obtain acarbose high-producing mutant strains. By knocking out the negative regulatory protein gene in the present invention, the expression of the acarbose biosynthesis gene can be promoted, and finally the acarbose production can be significantly improved. Compared with the starting strain, the fermentation yield of the high-yielding strain obtained by the invention is increased by 13% and 8% respectively, and the fermentation level of the laboratory shake flask reaches 3.62g/L and 3.47g/L respectively. |
| priorityDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 139.