http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112522126-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 |
| filingDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112522126-B |
| titleOfInvention | High-yielding microbial oil red yeast genetically engineered bacteria and its construction and culture method |
| abstract | The invention discloses a genetic engineering bacterium of high-yield microorganism Rhododendron oleifera and a construction and culture method thereof. In the present invention, a red yeast U13N3 with a deposit number of CCTCC NO: M 2019503 and a color mutation is used as the starting strain, and the functions of triacylglycerol lipase AOX2 and ATG15 are simultaneously deleted by designing a specific gRNA sequence and using a CRISPR/Cas9 gene editing system. , constructed a genetically engineered strain of high-yielding microbial Rhododendron oleifera. The red yeast genetically engineered bacteria of the present invention uses glycerol as a carbon source, and the intracellular oil content reaches a maximum of 0.854 g/g stem cells after fermentation for 120 hours, and the oil-producing ability is strong. The subsequent treatment process is simplified, and it is suitable for industrialized large-scale production. |
| priorityDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.