http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112521498-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-505 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-241 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P37-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 |
| filingDate | 2020-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112521498-B |
| titleOfInvention | TNF-alpha targeted humanized nano antibody and preparation and application thereof |
| abstract | The invention provides a humanized nano antibody targeting TNF-alpha, and the amino acid sequence of the humanized nano antibody is shown in any one of SEQ ID NO 1-SEQ ID NO 5.A novel phage library of the humanized nano antibody is designed and constructed, and a solid phase screening method is utilized to obtain the gene sequence of the humanized nano antibody for resisting TNF-alpha. Then, the nano antibody gene is cloned to a prokaryotic expression vector through gene operation, and BL21(DE3) is utilized to express and obtain high soluble nano antibody protein. The humanized nano antibody prepared by the invention has the advantages of small immunogenicity, good stability, long half-life, easy expression and easy coupling with other molecules, and can be used for diagnosis/imaging/tracing and treating inflammatory diseases. Therefore, the antibody has wide application prospect as an antibody drug. |
| priorityDate | 2020-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 230.