http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112501320-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 |
| filingDate | 2021-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112501320-B |
| titleOfInvention | Snake origin component rapid detection kit and application thereof |
| abstract | The invention provides a snake-derived component rapid detection kit and application thereof, and relates to the technical field of biological species identification. The invention firstly screens out a snake-derived universal internal standard gene JUN(Gen bank No.EF144048.1) The nucleotide sequence is shown as SEQ ID NO.1, is located on chromosome, has constant copy number in snake species, has no allelic variation, and can be used as a target gene for identifying snake origin. The gene is used as a target sequence to design a constant-temperature amplification primer, and a constant-temperature amplification reaction product is used for detecting the colloidal gold nucleic acid test strip. The constant temperature amplification reaction is combined with the detection of the colloidal gold nucleic acid test strip to form a rapid detection kit, the snake-derived components can be rapidly and sensitively detected, and the detection sensitivity can reach 0.8% (w/w). The kit has the advantages of simple use method, low cost, easy observation of reaction results and good specificity, and is very suitable for field real-time detection. |
| priorityDate | 2021-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.