http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112501310-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2020-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112501310-B |
| titleOfInvention | A LAMP detection primer set, kit and method for bovine-derived components |
| abstract | The invention discloses a LAMP detection primer set and a detection method for bovine-derived components. The method includes the following steps: 1) Designing and synthesizing primers; the primers are composed of an upstream internal primer FIP, a downstream internal primer BIP, an upstream external primer F3 and a downstream external primer B3, and the base sequences of the primers are as shown in SEQ ID No: 1 ～4; 2) Prepare DNA dilutions of bovine bison, dairy cow, yak and buffalo; 3) Prepare LAMP reaction system and set reaction conditions; 4) Carry out LAMP method specificity detection; 5) Carry out LAMP method sensitivity detection; 6) Determine whether the sample to be tested is of bovine origin by electrophoresis whether there is a ladder band. The method established by the invention has the advantages of rapidity, high efficiency, sensitivity, accuracy and the like, and has practical application value in the supervision and inspection and quarantine of animal-derived ingredients food, and is especially suitable for the promotion and application of basic-level laboratories and on-site detection. |
| priorityDate | 2020-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.