http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112501257-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6417 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112501257-B |
| titleOfInvention | Visualization sensor based on nucleic acid self-assembly enzyme-catalysis-free circRNA living cell imaging |
| abstract | The invention provides a visualization sensor based on nucleic acid self-assembly enzyme-catalysis-free circRNA living cell imaging, which comprises the following components: (1) the system comprises (1) a graphene oxide GO-based intracellular efficient delivery system, (2) a GO-based signal switch system, and (3) a CHA and HCR combined nucleic acid self-assembly double-signal amplification imaging system without enzyme catalysis. The invention completes the detection of target circRNA in living cells by the design of CHA and HCR hairpin probes and by using the biocompatibility and fluorescence quenching effect of GO. The problems that the circRNA detection process is complicated and sensitive visualization is difficult by traditional detection means such as PCR, sequencing and northblot are solved, and quick, high-sensitivity and high-selectivity living cell visualization detection of the circRNA is realized. The detection of circRNA as a biomarker is more accurate, real-time and efficient. |
| priorityDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.