http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112501151-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-01084 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 |
filingDate | 2020-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112501151-B |
titleOfInvention | Nitrile hydratase mutant and application thereof |
abstract | The invention discloses a nitrile hydratase mutant and application thereof, belonging to the technical field of biological engineering. The enzyme activities of single-point mutants L48D and L48H constructed on the No. 48 subunit in a nitrile hydratase amino acid motif are 750.26 +/-1.63U/mg and 820.01 +/-0.98U/mg respectively, and are obviously improved compared with the specific enzyme activity 220.30 +/-2.28U/mg of wild nitrile hydratase without modification. Wherein, the enzyme activities of the mutant L48D and the mutant L48H are respectively 3.4 times and 3.7 times of the wild type. The mutation of the two amino acid residues obviously improves the catalytic activity of nitrile hydratase, is beneficial to the production of fine chemicals such as nicotinamide in industry, improves the catalytic efficiency and reduces the production cost. |
priorityDate | 2020-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 341.