| abstract |
The present invention provides a colorectal cancer tumor suppressor gene, which is CPEB1 gene. The invention also provides a method for verifying the high DNA methylation of the promoter region of the tumor suppressor gene of colorectal cancer. Western blotting to detect protein levels, flow cytometry to detect cell apoptosis, CCK-8 assay and cell clone formation assay to evaluate cell proliferation and cloning ability, damage repair assay to evaluate cell migration ability, Transwell chamber assay to evaluate cell invasion ability, nude mouse Tumor experiments simulate the tumorigenic ability of CPEB1 gene in vivo and other experiments, which prove that CPEB1 gene is a new colorectal cancer tumor suppressor gene, and its promoter region has high DNA methylation sites, and the gene expression is in a low expression pattern. |