http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112458187-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d04c24c31981cd02a4883835d09014f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3128a7404e4870a9cfd62a92fee0f18b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 |
| filingDate | 2020-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10467246ddc32d2ec3a668e90461c860 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73aea472e329ce9030b365be8a4bc5c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d917aaf2b7f7ec43062c95a533c18971 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_720648f2574b2e3ba529bcf188a5ec10 |
| publicationDate | 2021-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112458187-A |
| titleOfInvention | HDA isothermal amplification reagent detection method for detecting pine wood nematode |
| abstract | The invention provides an HDA isothermal amplification kit for detecting pine wood nematodes and a detection method, wherein the kit mainly comprises the following reagents: reagent A: pine wood nematode tissue lysate; and (3) reagent B: HDA isothermal amplification buffer solution, wherein the buffer solution contains specific primers BxHDAF and BxHDAR; and (3) reagent C: HDA isothermal amplification reaction enzyme liquid contains helicase. And (3) detecting the amplified product through electrophoresis, and determining that the pine wood nematode exists in the detected sample when a target electrophoresis band appears, wherein the target electrophoresis band is 97 bp. The kit can be used for quickly, sensitively and accurately detecting and judging whether the nematode in the pine wood is the pine wood nematode according to the method provided by the invention. |
| priorityDate | 2020-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.