http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112458149-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 |
| filingDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112458149-B |
| titleOfInvention | Method for detecting activation degree of antibody drug on T cells by taking CD3 as target |
| abstract | The invention relates to the field of biological medicine, and particularly provides a method for detecting the activation degree of an antibody drug taking CD3 as a target spot on T cells, which comprises the following steps: constructing and screening engineering cell strains with stable expression; adjusting the cell density of the engineering cell strain, and performing cell plating; diluting the antibody drug taking CD3 as a target spot in a concentration gradient manner, and incubating for 4-5 hours; centrifuging and collecting the precipitate, fully lysing, mixing with luciferase substrate, reading fluorescence value, and plotting fluorescence value-drug concentration curve. The method evaluates the activation degree of the T cells by detecting the luciferase expression level induced and expressed by NFAT, shortens the detection period to 4-5 hours, saves the economic cost, has simple operation method and more accurate detection result without using fresh human blood and a detection kit, and can be widely applied to the detection of the activation degree of the T cells by the anti-CD3 monoclonal antibody or the bispecific antibody. |
| priorityDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 468.