http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112458080-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-136 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-178 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112458080-B |
| titleOfInvention | A siRNA fishing method for lncRNA LOC157273 |
| abstract | In order to overcome the problems of poor reliability and high screening cost in existing lncRNA siRNA screening and identification technology, the present invention provides a method for catching siRNA targeting lncRNA LOC157273, comprising the following steps: obtaining different lncRNA LOC157273 gene fragments ; Multiple lncRNA LOC157273 gene fragments are scattered and bound to the surface of the substrate to make a gene chip; prepare an siRNA probe library targeting the lncRNA LOC157273 gene; hybridize the gene chip with the siRNA probe library; use a gene chip scanner to scan the hybridized The gene chip was scanned to determine the positive point of hybridization; PCR was performed on the positive point on the gene chip to amplify and purify the hybridized lncRNA LOC157273 gene fragment; the hybridized lncRNA LOC157273 gene fragment was sequenced to obtain its gene sequence, according to The gene sequence of the hybridized lncRNA LOC157273 gene fragment was reversely deduced to the siRNA sequence. The siRNA fishing method provided by the present invention starts from experiments, and can more conveniently and accurately catch lncRNA target siRNAs, and the results are reliable, accurate and comprehensive. |
| priorityDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 104.