http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112458072-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-54 |
| filingDate | 2020-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112458072-B |
| titleOfInvention | Alkaline protease mutant and preparation thereof |
| abstract | The invention belongs to the technical field of bioengineering, and particularly relates to an alkaline protease mutant and a preparation method thereof. The invention extracts the genome DNA of the Bacillus clausii, obtains a wild alkaline protease gene sequence with an zymogen region through PCR amplification, randomly mutates the wild alkaline protease gene obtained through amplification through error-prone PCR, and obtains sixteen high-activity alkaline protease genes through high-throughput screening. The sixteen mutant genes are constructed into a recombinant vector and successfully expressed in bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis and bacillus clausii to obtain a recombinant strain with improved enzyme production activity, and a novel alkaline protease is further obtained by optimizing a fermentation process. |
| priorityDate | 2020-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 306.