| abstract |
The invention relates to a preparation method of an immobilized cold-resistant microbial inoculum for strengthening the denitrification performance of a cold wetland, which comprises the following steps: 1) enrichment culture of cold-resistant ammonia oxidizing bacteria by using an SBR method, 2) preparation of bacterial suspension, 3) fixation of cold-resistant ammonia oxidizing bacteria: compounding the Brazilian foxtail spirillum and the chlorella vulgaris to obtain a chlorella compound solution, then uniformly mixing the sodium alginate solution and the chlorella compound solution to be used as an embedding carrier, adding sludge biochar to form a gel, then adding a bacterial suspension into the gel, uniformly mixing, standing for 3-4 h, slowly dropwise adding the mixture into a cross-linking agent, and cross-linking for 18-24 h and then washing for 3 times by using deionized water; the immobilized bacteria-algae pellets prepared by the method are applied to cold wetlands in winter, so that the nitrogen and phosphorus removal capability of a wetland system under cold conditions is remarkably improved, and the problems of poor operation effect, poor pollutant treatment effect and the like of the artificial wetland in winter are remarkably improved. |