http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112430574-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1a2a3aea0ca9b824c17551d36c2308a |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2511-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2020-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffb800725dff416927e98c5b624f54f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8536a3b477608b2c8bd46c1f1b5f47c7 |
| publicationDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112430574-A |
| titleOfInvention | A method for constructing a magnetically controlled in vitro tumor array |
| abstract | A method for constructing a magnetically controlled in vitro tumor array belongs to the field of biomedicine. In order to solve the problems such as difficulty in producing relatively uniform tumor balls and complicated operation for three-dimensional tumor balls, the method includes: ultrasonically cleaning the woven nickel mesh, container and NdFeB magnet successively in detergent, distilled water and anhydrous ethanol, Blow dry, fix the nickel mesh on the bottom of the container and then fix it in the center of the magnet, and place it under ultraviolet light for 2-4 hours; collect the tumor cells in a centrifuge tube, concentrate by centrifugation, and mix 3 mL of the cell suspension with gadobutrol; Take 100-200 μL of cell culture solution into the container, wet the nickel mesh, add 1-2 mL of the mixture into the container, and the cells form a cell aggregate array on the nickel mesh; move the device into the cell incubator, and replace the cell culture every 12 hours. After 24 to 48 hours, tumor arrays were formed. The invention has simple operation, low manufacturing cost, and can produce tumor spheres with uniform size in high throughput. The prepared tumor spheres can be used in medical experiments. |
| priorityDate | 2020-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.