http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112430561-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_36218d26fee60aaadf720446e61da448 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cc4e13141d402ceb885f368dfa042348 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-77 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-15 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-77 |
| filingDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a6efb9662add415af7ebed121ae0c20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e04afc1a71a00f3d756c6cc565e4c09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c144e9cb16396a533e5448d2876a7a6e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3966bcd89e1ddfde8c816ea6f6dda8bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a74a5ca3f63bce9b75184e11aea3bb8b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6f7c823eb58a1e13fbf73f04e781392 |
| publicationDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112430561-A |
| titleOfInvention | A kind of efficient transformation method of stagnated Corynebacterium |
| abstract | The invention belongs to the technical field of molecular biology, in particular to a high-efficiency transformation method suitable for coryneform stasis. The transformation method focuses on systematically optimizing the electrotransformation conditions of Corynebacterium stasis, and improving the transformation efficiency by systematically optimizing factors such as medium and electric field strength, so as to realize the editing of the genome of Corynebacterium stasis, and achieve the purpose of constructing the target engineering bacteria. Control the initial optical density value and the OD 600nm parameter to be 0.29-0.31, transfer the above-mentioned seed solution into the optimized competent medium, and cultivate at 31-32°C and 249-252 rpm, so that the OD 600nm parameter reaches 0.7-1.1; When the OD 600nm value of competent cells was 0.7-1.1, the plasmid dosage of 1 μg, the ice bath time of 6 min, 10% glycerol, 1.8 KV, 5 ms, 3 consecutive electric shocks, and the recovery time of 3 h had the highest transformation efficiency, reaching the highest transformation efficiency. 331cfu/μg. The high-efficiency transformation method of coryneform stasis of the invention is simple in operation and high in transformation rate. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113755374-A |
| priorityDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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