http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112410399-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_165340c449d99165bb266b5b7c092fc1 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2326-12 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-28 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 |
| filingDate | 2020-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb12f7a463f707f932db4ca85b49920a |
| publicationDate | 2021-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112410399-A |
| titleOfInvention | High-sensitivity single-component TMB color developing solution and preparation method thereof |
| abstract | The invention discloses a high-sensitivity single-component TMB color developing solution, which comprises the following components: 0.001-20 g/L of polyvinyl alcohol, 0.16-30 g/L of xylose, 1-100 mM of citric acid, 1-20 mM of ethylene diamine tetraacetic acid, 1-10 mM of 30% aqueous hydrogen peroxide, 1-3 mM of TMB, 20-30 ml/L of DMSO, 3000.04% of proclin, 1-25 mM of disodium hydrogen phosphate and the volume of ultrapure water is up to 1L. A preparation method of high-sensitivity single-component TMB color developing solution comprises the following steps: the method comprises the following steps: preparing a reagent mother solution: 1.1: 50g/L of low-viscosity polyvinyl alcohol 1788: accurately weighing 5g of 1788 low-viscosity polyvinyl alcohol in a beaker, adding a proper amount of ultrapure water, placing in a constant-temperature water bath kettle at 65 ℃, continuously stirring through a glass rod to uniformly disperse particles, continuously preserving heat for 2 hours after dissolution, fixing the volume of the ultrapure water to 100ml, and filtering by using a 0.45 mu m filter membrane. The invention has the characteristic of low detection background, effectively improves the detectable rate of low-concentration samples, does not need to mix the traditional liquid A and the traditional liquid B, provides convenience for the operation of users, and makes the invention have prominent progress on the whole. |
| priorityDate | 2020-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.